Coding
Part:BBa_K2074028:Design
Designed by: Ruicheng Dai Group: iGEM16_FAFU-CHINA (2016-10-09)
Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 986
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1378
Illegal NgoMIV site found at 3631 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Illegal BsaI.rc site found at 184
Illegal BsaI.rc site found at 2071
Illegal BsaI.rc site found at 2611
Illegal BsaI.rc site found at 2920
Illegal BsaI.rc site found at 3487
Illegal BsaI.rc site found at 4150
Illegal SapI.rc site found at 1188
Illegal SapI.rc site found at 1230
Design Notes
To identify there is no restriction enzyme cutting site which we choosed.
Source
DNA from a synthesis company.